6/28/2023 0 Comments Pcdna3 1 snapgene![]() ![]() PaeR7I does not recognize the sequence CTCTCGAG.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. Sticky ends from different EcoO109I sites may not be compatible. Prolonged incubation with NdeI may lead to removal of additional nucleotides. Sticky ends from different Bpu10I sites may not be compatible.
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